Effect of Temperature on Symptoms Expression and Viral RNA Accumulation in Groundnut Bud Necrosis Virus Infected Vigna unguiculata
نویسندگان
چکیده
منابع مشابه
Immunodiagnosis of groundnut and watermelon bud necrosis viruses using polyclonal antiserum to recombinant nucleocapsid protein of Groundnut bud necrosis virus.
In vitro gene expression strategy was used for the production of polyclonal antiserum to the nucleocapsid protein (NP) of Groundnut bud necrosis virus (GBNV). The GBNV NP gene from cowpea isolate was cloned into 6x His-tagged UA cloning vector and expressed in Escherichia coli [M15] cells. The fusion protein was detected in insoluble fraction and was purified by using Ni-NTA agarose resin. The ...
متن کاملBud necrosis of groundnut (Arachis hypogaea) in India caused by tomato spotted wilt virus*
Chlorotic ringspots or chlorotic leaf specking, terminal bud necrosis, axillary shoot proliferation and severe stunting of groundnut (Arachis hypogaea) were shown to be caused by tomato spotted wilt virus (TSWV). Cowpea (Vigna unguiculata cv. C-152) was found to be a good assay host. TSWV remained infective in buffered sap of groundnut at a dilution of 10-2’5, after storage for 4 h at room temp...
متن کاملCorrigendum: Effect of temperature on the pathogenesis, accumulation of viral and satellite RNAs and on plant proteome in peanut stunt virus and satellite RNA-infected plants
[This corrects the article on p. 903 in vol. 6, PMID: 26579153.].
متن کاملEffect of temperature on the pathogenesis, accumulation of viral and satellite RNAs and on plant proteome in peanut stunt virus and satellite RNA-infected plants
Temperature is an important environmental factor influencing plant development in natural and diseased conditions. The growth rate of plants grown at C27°C is more rapid than for plants grown at 21°C. Thus, temperature affects the rate of pathogenesis progression in individual plants. We have analyzed the effect of temperature conditions (either 21°C or 27°C during the day) on the accumulation ...
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ژورنال
عنوان ژورنال: Iranian Journal of Biotechnology
سال: 2018
ISSN: 2322-2921
DOI: 10.21859/ijb.1846